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volume of. It is also a part of the restriction modification system. The / in the sequence indicates which phosphodiester bond the enzyme will break in the DNA molecule. Bonatelli IA, Carstens BC, Moraes. The, eco part of the enzyme's name originates from the species from which it was isolated, while the R represents the particular strain, in this case RY13. ..

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Simonii Using Restriction-Site Associated DNA Sequencing. Quality Safety Quality Control Assays Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for. "Refinement of Eco RI endonuclease crystal structure: a revised protein chain tracing". (2015) Two new phragmotic ant species from Africa: morphology and next-generation sequencing solve a caste association problem in the genus Carebara Westwood. Tertiary and quaternary structure edit, the enzyme is a homodimer of a 31 kilodalton subunit consisting of one globular domain of the / architecture. NEBuffer EcoRI -20 10 X, product Categories: Restriction Endonucleases: C-G, Time-Saver Qualified Restriction Enzymes, applications: Fast Cloning: Accelerate your cloning workflows with reagents from NEB, Restriction Enzyme Digestion.

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volume of. It is also a part of the restriction modification system. The / in the sequence indicates which phosphodiester bond the enzyme will break in the DNA molecule. Bonatelli IA, Carstens BC, Moraes. The, eco part of the enzyme's name originates from the species from which it was isolated, while the R represents the particular strain, in this case RY13. ..

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Ec oRI recognition site with cutting pattern indicated by a green line. Proceedings of the National Academy of Sciences of the United States of America. FAQs Tech Tips Protocols Manuals Other Tools Resources Selection Charts Usage Guidelines Tips Troubleshooting Guides Interactive Tools Citations Tong C, Li H, Wang Y, Li X, Ou J, Wang D, Xu H, Ma C, Lang X, Liu G, Zhang B, Shi. Restriction Enzyme Digestion Problem: DNA Smear on Agarose Gel.

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Coli strain that carries the cloned EcoRI gene from. The following file naming structure is used to name these document files: Product Number_Size_Version_Lot Number Safety Data Sheets The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely. Eco, rI creates 4 nucleotide sticky ends with 5' end overhangs of aatt.